Field evaluation of BD FACSPrest for haemoglobin and CD4 measurement
Date
2017
Authors
Chaba, Linda
Kingwara, Leonard
Muriungi, Henry
Journal Title
Journal ISSN
Volume Title
Publisher
Scientific and Academic Publishing Co.
Abstract
Background:Measurements of CD4 and haemoglobin are used to determine the immunological state and information about disease progression for HIV-infected patients. Use of BD FACS Presto™ point of care (POC) device for CD4 and haemoglobin (Hb) determination can significantly improve access, uptake and coverage of laboratory services and hence management of HIV-infected patients in resource-limited settings. This study evaluated the relative bias in CD4 and Hb measurements using BD FACSPresto™ system compared to BD FACSCalibur™ CD4 analyser and Mindray BC-5380 haematology analyser respectively based on venous and capillary blood samples in a clinical hospital setting. Methods:Venous and capillary blood samples were used to determine CD4 counts and Hb levels among HIV-1 infected patients. The samples were analysed on the BD FACSPresto™ and results compared against BD FACSCalibur™ and Mindray BC-5380 for CD4 and haematology analyser respectively. Results: Results for absolute CD4 counts in both venous and capillary blood showed a high correlation (R2 = 0.922, P< 0.001) when they were analysed on BD FACSPresto™ and BD FACSCalibur™ machines. Overall, the mean difference in absolute CD4 count was 77.16 cells/mL (95%CI: 49.89, 104.42, p<0.01) when analysed on two platforms. The BD FACSCalibur™ gave a higher mean of absolute CD4 count (834.38 cells/ml) compared to BD FACSPresto™ (757.23 cells/ml) when venous sample type is used. There was a significant mean difference of Hb levels at 0.31 (P <0.001) between the two sample types when analysed on BD FACSPresto™ and Mindray BC-5380 haematology analyser. In addition, there was a high correlation (R2 = 0.920, P< 0.001) of Hb level measurements between the BD FACSPresto™ and Mindray BC-5380 haematology analyser.Conclusion: The BD FACSPresto performed satisfactorily in comparison to the conventional reference standard technologies. Venous and capillary blood sample types showed a high correlation when analysed for absolute CD4 count and Hb using BD FACSPresto™, BD FACSCalibur™ and Mindray BC-5380 haematology analyser. BD FACSPresto capillary platform can be used interchangeably with BD FACSCalibur™ venous platform for CD4 and Mindray BC-5380 for Hb measurement in resource limited settings to increase access and uptake of laboratory services.
Description
Keywords
FACSPresto™,, Immunology,, Laboratory services, HIV-1, haemoglobin, CD4 measurement